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psih1puro vector  (Addgene inc)


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    Structured Review

    Addgene inc psih1puro vector
    Psih1puro Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/psih1+puro+vector/pSIH1-puro-control+shRNA+(Plasmid+%2326597)/pm41735312-295-1-4
    Average 94 stars, based on 32 article reviews
    psih1puro vector - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    shRNA:

    Article Title: DLGAP1-AS2–Mediated Phosphatidic Acid Synthesis Activates YAP Signaling and Confers Chemoresistance in Squamous Cell Carcinoma
    Article Snippet: Virus production and cell infection Full-length cDNAs were constructed using PCR amplification and subsequently inserted into the pLVX-IRES-neo vector (#632184; Clontech). .. The short hairpin RNA (shRNA) sequences were cloned into the pSIH1-puro vector (#26597; Addgene). .. The single-guide RNA (sgRNA) sequences were cloned into LentiCRISPR v.2 (#52961; Addgene) backbone.

    Article Title: MAFB promotes the malignant phenotypes by IGFBP6 in esophageal squamous cell carcinomas.
    Article Snippet: Esophageal squamous cell carcinoma (ESCC) is one of the most common malignant diseases in the world.. Although the somatic alterations have been fully identified, there are still no targeted drugs at present.. Our previous studies revealed that loss of grand H3K27me3 domains mediated transcriptional activation of a series of genes in ESCC.

    Article Title: m6A demethylase FTO stabilizes LINK-A to exert oncogenic roles via MCM3-mediated cell cycle progression and HIF-1α activation
    Article Snippet: The full-length cDNA of MCM3 was cloned into the plasmid PEZ-LV105 (M0227; GeneCopoeia, Guangzhou, Guangdong, China). .. The shRNA sequences were cloned into the pSIH1-puro vector (#26597; Addgene, Cambridge, MA, USA). .. The sgRNA sequences were cloned into the LentiCRISPR v.2 (#52961; Addgene) backbone.

    Article Title: m 6 A Demethylase FTO Stabilizes LINK-A to Exert Oncogenic Roles via MCM3-Mediated Cell Cycle Progression and HIF-1α Activation
    Article Snippet: The full-length cDNA of MCM3 was cloned into the plasmid PEZ-LV105 (M0227; GeneCopoeia, Guangzhou, Guangdong, China). .. The shRNA sequences were cloned into the pSIH1-puro vector (#26597; Addgene, Cambridge, MA, USA). .. The sgRNA sequences were cloned into the LentiCRISPR v.2 (#52961; Addgene) backbone.

    Article Title: m 6 A demethylase FTO stabilizes LINK-A to exert oncogenic roles via MCM3-mediated cell-cycle progression and HIF-1α activation.
    Article Snippet: The full-length cDNA of MCM3 was inserted into the PEZ-LV105 vector (M0227; GeneCopoeia, Guangzhou, China). .. The shRNA sequences were inserted into the pSIH1-puro vector (#26597; Addgene, MA, USA). .. The single-guide RNA (sgRNA) sequences were inserted into the LentiCRISPR v2 (#52961; Addgene) backbone.

    Article Title: DLGAP1-AS2-Mediated Phosphatidic Acid Synthesis Confers Chemoresistance via Activation of YAP Signaling
    Article Snippet: Full-length cDNAs were constructed using PCR amplification and subsequently inserted into the pLVX-IRES-neo vector (#632184; Clontech, CA, USA). .. The shRNA sequences were cloned into the pSIH1-puro vector (#26597; Addgene, MA, USA). .. Lentiviruses were produced in 293T cells with a second-generation packaging system containing psPAX2 (#12260; Addgene) and pMD2.G (#12259; Addgene).

    Article Title: Enhancer demethylation-regulated gene score identified molecular subtypes, inspiring immunotherapy or CDK4/6 inhibitor therapy in oesophageal squamous cell carcinoma
    Article Snippet: .. The shRNA sequences were inserted into the pSIH1-puro vector (#26597; Addgene, Cambridge, MA, USA) (RRID: Addgene_26597). ..

    Article Title: Targeting de novo pyrimidine synthesis confers vulnerability to copper-mediated ATR inactivation in PARP inhibitor-resistant ovarian cancer
    Article Snippet: Lentiviruses were produced in 293T cells using a second-generation packaging system involving psPAX2 (#12260, Addgene, MA, USA) and pMD2.G (#12259, Addgene) plasmids. .. The pSIH1-puro vector (#26597, Addgene) was used to incorporate the shRNA sequences. .. The sgRNA sequences targeting human FDX1 were inserted into the LentiCRISPR v.2 (#52961, Addgene) backbone.

    Clone Assay:

    Article Title: DLGAP1-AS2–Mediated Phosphatidic Acid Synthesis Activates YAP Signaling and Confers Chemoresistance in Squamous Cell Carcinoma
    Article Snippet: Virus production and cell infection Full-length cDNAs were constructed using PCR amplification and subsequently inserted into the pLVX-IRES-neo vector (#632184; Clontech). .. The short hairpin RNA (shRNA) sequences were cloned into the pSIH1-puro vector (#26597; Addgene). .. The single-guide RNA (sgRNA) sequences were cloned into LentiCRISPR v.2 (#52961; Addgene) backbone.

    Article Title: MAFB promotes the malignant phenotypes by IGFBP6 in esophageal squamous cell carcinomas.
    Article Snippet: Esophageal squamous cell carcinoma (ESCC) is one of the most common malignant diseases in the world.. Although the somatic alterations have been fully identified, there are still no targeted drugs at present.. Our previous studies revealed that loss of grand H3K27me3 domains mediated transcriptional activation of a series of genes in ESCC.

    Article Title: m6A demethylase FTO stabilizes LINK-A to exert oncogenic roles via MCM3-mediated cell cycle progression and HIF-1α activation
    Article Snippet: The full-length cDNA of MCM3 was cloned into the plasmid PEZ-LV105 (M0227; GeneCopoeia, Guangzhou, Guangdong, China). .. The shRNA sequences were cloned into the pSIH1-puro vector (#26597; Addgene, Cambridge, MA, USA). .. The sgRNA sequences were cloned into the LentiCRISPR v.2 (#52961; Addgene) backbone.

    Article Title: m 6 A Demethylase FTO Stabilizes LINK-A to Exert Oncogenic Roles via MCM3-Mediated Cell Cycle Progression and HIF-1α Activation
    Article Snippet: The full-length cDNA of MCM3 was cloned into the plasmid PEZ-LV105 (M0227; GeneCopoeia, Guangzhou, Guangdong, China). .. The shRNA sequences were cloned into the pSIH1-puro vector (#26597; Addgene, Cambridge, MA, USA). .. The sgRNA sequences were cloned into the LentiCRISPR v.2 (#52961; Addgene) backbone.

    Article Title: DLGAP1-AS2-Mediated Phosphatidic Acid Synthesis Confers Chemoresistance via Activation of YAP Signaling
    Article Snippet: Full-length cDNAs were constructed using PCR amplification and subsequently inserted into the pLVX-IRES-neo vector (#632184; Clontech, CA, USA). .. The shRNA sequences were cloned into the pSIH1-puro vector (#26597; Addgene, MA, USA). .. Lentiviruses were produced in 293T cells with a second-generation packaging system containing psPAX2 (#12260; Addgene) and pMD2.G (#12259; Addgene).

    Plasmid Preparation:

    Article Title: DLGAP1-AS2–Mediated Phosphatidic Acid Synthesis Activates YAP Signaling and Confers Chemoresistance in Squamous Cell Carcinoma
    Article Snippet: Virus production and cell infection Full-length cDNAs were constructed using PCR amplification and subsequently inserted into the pLVX-IRES-neo vector (#632184; Clontech). .. The short hairpin RNA (shRNA) sequences were cloned into the pSIH1-puro vector (#26597; Addgene). .. The single-guide RNA (sgRNA) sequences were cloned into LentiCRISPR v.2 (#52961; Addgene) backbone.

    Article Title: MAFB promotes the malignant phenotypes by IGFBP6 in esophageal squamous cell carcinomas.
    Article Snippet: Esophageal squamous cell carcinoma (ESCC) is one of the most common malignant diseases in the world.. Although the somatic alterations have been fully identified, there are still no targeted drugs at present.. Our previous studies revealed that loss of grand H3K27me3 domains mediated transcriptional activation of a series of genes in ESCC.

    Article Title: Targeting de novo pyrimidine synthesis confers vulnerability to copper-mediated ATR inactivation in PARP inhibitor-resistant ovarian cancer
    Article Snippet: Lentiviruses were produced in 293T cells using a second-generation packaging system involving psPAX2 (#12260, Addgene, MA, USA) and pMD2.G (#12259, Addgene) plasmids. .. The pSIH1-puro vector (#26597, Addgene) was used to incorporate the shRNA sequences. .. The sgRNA sequences targeting human FDX1 were inserted into the LentiCRISPR v.2 (#52961, Addgene) backbone.

    Control:

    Article Title: MAFB promotes the malignant phenotypes by IGFBP6 in esophageal squamous cell carcinomas.
    Article Snippet: Esophageal squamous cell carcinoma (ESCC) is one of the most common malignant diseases in the world.. Although the somatic alterations have been fully identified, there are still no targeted drugs at present.. Our previous studies revealed that loss of grand H3K27me3 domains mediated transcriptional activation of a series of genes in ESCC.



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